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Flow‐injection chemiluminescence determination of acetylsalicylic acid based on its enhancing effect on the lucigenin–hydrogen peroxide system
Author(s) -
Wabaidur S. M.,
Alam S. M.,
Alothman Z. A.,
Eldesoky Gaber
Publication year - 2014
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.2609
Subject(s) - lucigenin , chemiluminescence , chemistry , hydrogen peroxide , chromatography , relative standard deviation , detection limit , flow injection analysis , luminol , standard curve , organic chemistry , enzyme , superoxide
A sensitive flow‐injection chemiluminescence method for the determination of acetylsalicylic acid is described. It is based on the enhanced chemiluminescent emission of the alkaline lucigenin–H 2 O 2 system by acetylsalicylic acid. The difference in chemiluminescent intensity of alkaline lucigenin–H 2 O 2 in the presence of acetylsalicylic acid from that in the absence of acetylsalicylic acid was linear at acetylsalicylic acid concentrations in the range of 0.0029–47.37 µg/mL, with detection and quantification limits of 0.0011 and 0.0029 µg/mL , respectively. The correlation coefficient of the working curve was 0.9983. The relative standard deviation ( n = 10) for 25 µg/mL acetylsalicylic acid is 1.95%. All experimental parameters were optimized. The method was successfully applied to the determination of acetylsalicylic acid in pharmaceutical preparations. The recovery results obtained by the method were satisfactory. Copyright © 2013 John Wiley & Sons, Ltd.