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Synthesis of a novel fluorescent probe based on 7‐nitrobenzo‐2‐oxa‐1,3‐diazole skeleton for the rapid determination of vitamin B 12 in pharmaceuticals
Author(s) -
Shang Zhuo Bin,
Wen Ya Juan,
Yan Xiao Qing,
Sun Huan He,
Wang Yu,
Jin Wei Jun
Publication year - 2014
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.2588
Subject(s) - fluorescence , chemistry , detection limit , yield (engineering) , vitamin , quenching (fluorescence) , vitamin b , linear range , analytical chemistry (journal) , chromatography , nuclear chemistry , materials science , biochemistry , physics , quantum mechanics , metallurgy
ABSTRACT A new fluorescent probe, 4‐ N , N ‐di(2‐hydroxyethyl)imino‐7‐nitrobenzo‐2‐oxa‐1,3‐diazole (HINBD) was synthesized in a single step with reasonably good yield. The water‐soluble HINBD emits strongly in the visible region (λ ex = 479 nm, λ em = 545 nm) and is stable over a wide range of pH values. It was found that vitamin B 12 (VB 12 ) had the ability to quench the fluorescence of HINBD, and the quenched fluorescence intensity was proportional to the concentration of VB 12 . A method for VB 12 determination based on the quenching fluorescence of HINBD was thus established. Interference effects of various substances, including sugars, vitamins, amino acids, inorganic cations and some organic substances have been studied. Under optimal conditions, the linear range is 0.0–2.4 × 10 –5 mol/L. The determination limit is 8.3 × 10 –8 mol/L. The method was applied to measure VB 12 in pharmaceutical preparations with satisfactory results. Copyright © 2013 John Wiley & Sons, Ltd.