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Electrophoresis–chemiluminescence detection of phenols catalyzed by hemin
Author(s) -
Shu Lu,
Zhu Jinkun,
Wang Qingjiang,
He Pingang,
Fang Yuzhi
Publication year - 2014
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.2584
Subject(s) - hemin , chemistry , hydrogen peroxide , detection limit , luminol , chemiluminescence , phenol , catalysis , phenols , reagent , aqueous solution , ammonium hydroxide , capillary electrophoresis , nuclear chemistry , inorganic chemistry , chromatography , organic chemistry , heme , enzyme
Based on the catalytic activity of hemin, an efficient biocatalyst, an indirect capillary electrophoresis–chemiluminescence (CE‐CL) detection method for phenols using a hemin–luminol–hydrogen peroxide system was developed. Through a series of static injection experiments, hemin was found to perform best in a neutral solution rather than an acidic or alkaline medium. Although halide ions such as Br − and F − could further enhance the CL signal catalyzed by hemin, it is difficult to apply these conditions to this CE‐CL detection system because of the self‐polymerization of hemin, as it hinders the CE process. The addition of concentrated ammonium hydroxide to an aqueous/dimethyl sulfoxide solution of hemin–luminol afforded a stable CE‐CL baseline. The indirect CE‐CL detection of five phenols using this method gave the following limits of detections: 4.8 × 10 −8 mol/L ( o ‐sec‐butylphenol), 4.9 × 10 −8 mol/L ( o ‐cresol), 5.4 × 10 −8 mol/L ( m ‐cresol), 5.3 × 10 −8 mol/L (2,4‐dichlorophenol) and 7.1 × 10 −8 mol/L (phenol). Copyright © 2013 John Wiley & Sons, Ltd.