Sensitive determination of 2‐methoxyestradiol in pharmaceutical preparations and serum samples using flow injection chemiluminescence | Zendy

Yao Hanchun | Zendy; Zhang Min | Zendy; Zeng Wenyuan | Zendy; Zeng Xiaoying | Zendy; Zhang Zhenzhong | Zendy

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Sensitive determination of 2‐methoxyestradiol in pharmaceutical preparations and serum samples using flow injection chemiluminescence

Author(s) -

Yao Hanchun,

Zhang Min,

Zeng Wenyuan,

Zeng Xiaoying,

Zhang Zhenzhong

Publication year - 2014

Publication title -

luminescence

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.428

H-Index - 45

eISSN - 1522-7243

pISSN - 1522-7235

DOI - 10.1002/bio.2538

Subject(s) - chemiluminescence , 2 methoxyestradiol , detection limit , chemistry , sodium hydroxide , potassium ferricyanide , chromatography , relative standard deviation , potassium hydroxide , calibration curve , flow injection analysis , analytical chemistry (journal) , inorganic chemistry , biochemistry , in vitro , organic chemistry

A rapid and sensitive flow injection chemiluminescence (FI–CL) method is described for the determination of 2‐methoxyestradiol (2ME) based on enhancement of the CL intensity from a potassium ferricyanide–calcein system in sodium hydroxide medium. The optimum conditions for the CL emission were investigated. Under optimized conditions, a linear calibration graph was obtained over the range 1.0 × 10 ‐8 to 1.0 × 10 ‐6 mol/L ( r = 0.998) 2ME with a detection limit (3σ) of 5.4 × 10 ‐9 mol/L. The relative standard deviation (RSD) for 5.0 × 10 ‐7 mol/L 2ME was 1.7%. As a preliminary application, the proposed method was successfully applied to the determination of 2ME in injection solutions and serum samples. The possible CL mechanism was also proposed. Copyright © 2013 John Wiley & Sons, Ltd.

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