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Flow‐injection chemiluminescence determination of gentamicin: optimization by central composite design
Author(s) -
Iranifam M,
Hasanzadeh A,
Fathinia M,
Khataee A R,
Mousavi S A
Publication year - 2014
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.2533
Subject(s) - chemiluminescence , gentamicin sulfate , luminol , chemistry , central composite design , chromatography , gentamicin , relative standard deviation , sulfate , flow injection analysis , linear range , detection limit , analytical chemistry (journal) , nuclear chemistry , response surface methodology , organic chemistry , biochemistry , antibiotics
A simple and sensitive flow‐injection chemiluminescence (CL) method has been developed for the determination of gentamicin sulfate. The method is based on the inhibitory effect of gentamicin on the CL emission accompanying oxidation of luminol by H 2 O 2 in an alkaline medium in the presence of Cu(II) as a catalyst. Inhibition was caused by the formation of a strong complex between analyte and the catalyst. Experimental variables, including the concentrations of luminol (µmol/L), H 2 O 2 (mol/L), Cu(II) (mol/L) and NaOH (mol/L), were optimized using a central composite design. Under optimum conditions, the plot of CL intensity versus gentamicin concentration was found to have two linear ranges. One range was at low concentrations from 1.0 to 10.0 mg/L and the other was from 10.0 to 30.0 mg/L. Precision was calculated by analyzing samples containing 5.0 mg/L gentamicin ( n = 11) and the relative standard deviation (RSD) was 1.7%. Also, a high injection throughput of 120 samples/h was achieved. This method was successfully applied to the determination of gentamicin sulfate in pharmaceutical formulations and water samples. Copyright © 2013 John Wiley & Sons, Ltd.