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Sensitive determination of DNA based on the interaction between prulifloxacin–terbium(III) complex and DNA
Author(s) -
Wu Ting,
Fang Biyun,
Chang Lin,
Liu Min,
Chen Fang
Publication year - 2013
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.2453
Subject(s) - terbium , intercalation (chemistry) , fluorescence , dna , ternary complex , analytical chemistry (journal) , chemistry , detection limit , ternary operation , luminescence , materials science , chromatography , inorganic chemistry , biochemistry , optics , physics , programming language , computer science , optoelectronics , enzyme
A simple spectrofluorimetric method is described for the determination of DNA, based on its enhancement of the fluorescence intensity of prulifloxacin (PUFX)–Tb 3+ . The luminescence intensity of the PUFX–Tb 3+ complex increased up to 10‐fold after adding DNA. The excitation and emission wavelengths were 345 and 545 nm, respectively. Under optimum conditions, variations in the fluorescence intensity showed a good linear relationship with the concentration of hsDNA in the range of 3.0 × 10 ‐9 to 1.0 × 10 ‐6 g/mL, with a correlation coefficient ( R ) of 0.997, and the detection limit was 2.1 × 10 ‐9 g/mL. The method was successfully applied to the determination of DNA in synthetic samples, and recoveries were in the range 97.3–102.0%. The mechanism of fluorescence enhancement of the PUFX–Tb 3+ complex by DNA is also discussed. The mechanism may involve formation of a ternary complex mainly by intercalation binding together with weak electrostatic interaction, which will increase the energy transition from ligand to Tb 3+ , increasing the rigidity of the complex, and decreasing the radiationless energy loss through O–H vibration of the H 2 O molecule in the PUFX–Tb 3+ compl+osed method is not only more robust and friendly to the environment, but also of relatively higher sensitivity. Copyright © 2013 John Wiley & Sons, Ltd.