Premium
Flow‐injection chemiluminescence determination of diazepam by oxidation with N ‐bromosuccinimide
Author(s) -
Han Suqin,
Jia Shize,
Guo Liang
Publication year - 2012
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.2452
Subject(s) - chemiluminescence , n bromosuccinimide , diazepam , chemistry , dichlorofluorescein , detection limit , chromatography , flow injection analysis , relative standard deviation , spectrophotometry , analytical chemistry (journal) , pharmacology , organic chemistry , halogenation , medicine , antioxidant
A rapid and sensitive flow‐injection chemiluminescence (FI–CL) method is described for the determination of diazepam based on its reaction with N ‐bromosuccinimide (NBS) in alkaline medium in the presence of dichlorofluorescein (DCF) as an effective energy‐transfer agent. Under optimum conditions, the proposed method allowed the measurement of diazepam over the range of 2.0 × 10 −6 to 2.0 × 10 −4 mol/L with a detection limit of 5.0 × 10 −7 mol/L. The relative standard deviation for 11 parallel measurements of 2.0 × 10 −5 mol/L diazepam was 2.1%. The method was applied satisfactorily for the determination of diazepam in pharmaceutical preparations, and the results agree well with those obtained by spectrophotometry. The use of the proposed system for the determination of diazepam in urine and plasma samples was also tested. The possible mechanism of the chemiluminescence reaction is discussed briefly. Copyright © 2012 John Wiley & Sons, Ltd.