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Study on the ternary system of MoO 4 2‐ –enzyme–PdCl 2 by resonance Rayleigh scattering, second‐order scattering and frequency‐doubling scattering spectra and its analytical application
Author(s) -
Wang Yaqiong,
Liu Shaopu,
Liu Zhongfang,
Hu Xiaoli,
Xue Jiaxing,
Tian Jing
Publication year - 2012
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.2410
Subject(s) - rayleigh scattering , scattering , chemistry , ternary operation , lyso , resonance (particle physics) , analytical chemistry (journal) , spectral line , ternary complex , atomic physics , physics , optics , chromatography , enzyme , detector , scintillator , biochemistry , astronomy , computer science , programming language
In pH 4.0 Britton–Robinson buffer medium, PdCl 2 was able to react with enzymes (EZ) such as lysozyme (LYSO) and papain (PAP) to form a coordination complex (EZ–PdCl 2 ), which further reacted with MoO 4 2‐ to form a ternary complex (MoO 4 2‐ –EZ–PdCl 2 ). As a result, the absorption and fluorescence spectra changed; new spectra of resonance Rayleigh scattering (RRS), second‐order scattering (SOS) and frequency‐doubling scattering (FDS) appeared and their intensities were enhanced greatly. The maximum RRS, SOS and FDS wavelengths of two ternary complexes were located at 310, 560 and 350 nm, respectively. The increments of scattering intensity were directly proportional to the concentrations of EZ within certain ranges. The detection limits (3σ) of LYSO and PAP were 4.5 and 14.0 ng/mL (RRS method), 9.6 and 57.8 ng/mL (SOS method), and 5.2 and 106.0 ng/mL (FDS method). Taking the MoO 4 2‐ –LYSO–PdCl 2 system, which was more sensitive, as an example, the effects of coexisting substances were evaluated. The methods showed excellent selectivity. Accordingly, new rapid, convenient, sensitive and selective scattering methods for the determination of LYSO and PAP were proposed and applied to determine LYSO in egg white with satisfactory results. The reaction mechanism and basis of the enhancement of scattering were discussed. Copyright © 2012 John Wiley & Sons, Ltd.