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The effect of Hg 2+ on the bioluminescence of Photobacterium leiognathi
Author(s) -
Li Ming,
Wang Jingxue,
Lin Hong
Publication year - 2012
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.2391
Subject(s) - bioluminescence , luciferase , photobacterium , chemistry , luminescence , cell growth , photobacterium phosphoreum , microbiology and biotechnology , bromide , gene expression , biochemistry , transfection , toxicity , biology , gene , bacteria , vibrio , genetics , physics , inorganic chemistry , optoelectronics , organic chemistry
Photobacteria were used as test objects for rapid monitoring of ecotoxicants. Specific inhibitory effects of Hg 2+ on bioluminescence and cell growth as well as the lux gene expression of Photobacterium leiognathi were studied. The 3‐(4,5‐Dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide reduction assay was used to evaluate cellular proliferation and mortality. The luminescent inhibition effect on P. leiognathi cells was found to increase as cellular mortality increased; y = 0.744x ‐ 4.8916, R² = 0.9794. However, this trend was not observed in cell growth processes. Quantitation of lux mRNAs by semi‐quantitative RT‐qPCR indicated that increases and decreases in luciferase mRNA integral level coincided with changes in luminescence intensity (R 2 = 0.93). Addition of Hg 2+ changed luminescence but without concomitant changes in extractable luciferase activity. Nevertheless, the presence of Hg 2+ changed lux gene expression. This phenomenon requires further research. Copyright © 2012 John Wiley & Sons, Ltd.