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Effects of the interaction between Na 2 Wo 4 ‐6‐benzylaminopurine anionic chelate and rhodamine 6G on the resonance Rayleigh scattering and fluorescence spectra and their analytical applications
Author(s) -
Li Xiaoyan,
Shang Zhou,
Wei He,
Yang Jidong
Publication year - 2012
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.2380
Subject(s) - rhodamine 6g , fluorescence , analytical chemistry (journal) , chemistry , rayleigh scattering , ternary operation , resonance (particle physics) , quenching (fluorescence) , ion , fluorescence spectroscopy , buffer solution , scattering , chromatography , atomic physics , optics , physics , organic chemistry , computer science , programming language
In pH 4.99‐6.06 Britton‐Robinson (BR) buffer medium, 6‐benzylaminopurine (6‐BA) reacted with Na 2 WO 4 to form 1:1 anionic chelate (6‐BA·WO 4 ) 2‐ , which further reacted with rhodamine 6G to form ternary ion complexes at room temperature. This resulted in a significant enhancement of resonance Rayleigh scattering (RRS) with a maximum RRS wavelength of 316 nm. Meanwhile, the fluorescence of the solution was quenched and excitation ( λ ex ) and emission ( λ em ) wavelengths of the fluorescence were 290 and 559 nm, respectively. Intensities of RRS enhancing (Δ I RRS ) and fluorescence quenching (Δ I F ) were directly proportional to concentrations of 6‐BA. As a result, RRS and fluorescence quenching for determination of trace amounts of 6‐BA were developed. Under optimal conditions, linear ranges and detection limits of the two methods were 0.05‐15.00 µg/mL and 8.2 ng/mL (RRS), 0.50‐15.00 µg/mL and 17.0 ng/mL, respectively. It was found that the RRS method was superior to fluorescence quenching. The influence of these methods were investigated and results showed that RRS had good selectivity. RRS was applied to determine 6‐BA in vegetable samples with satisfactory results. Furthermore, the reaction mechanisms of the ternary ion‐association system are discussed. In addition, the polarization experiment revealed that the resonance light scattering (RLS) peak of Na 2 WO 4 ‐6‐BA‐R6G consisted mainly of depolarized resonance fluorescence and resonance scattering. It was speculated that light emission fluorescence energy ( E L ) transformed into resonance light scattering energy ( E RLS ), which was a key reason for enhancement of RRS. Copyright © 2012 John Wiley & Sons, Ltd.