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Indirect electrochemiluminescence detection of lysine and histidine separated by capillary electrophoresis based on charge displacement
Author(s) -
Yu Huan,
Xu Lei,
You Tianyan
Publication year - 2012
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.2367
Subject(s) - capillary electrophoresis , electrochemiluminescence , histidine , chromatography , electrophoresis , chemistry , phosphate buffered saline , lysine , detection limit , analytical chemistry (journal) , amino acid , biochemistry
Indirect electrochemiluminescence (ECL) detection was applied for the analysis of lysine (Lys) and histidine (His) separated by capillary electrophoresis (CE). With the most effective electrophoretic buffer system, which contained 15 mM phosphate buffer (pH = 5.8) and 0.5 mM Tripropylamine (TPA), fast separation of the two basic amino acids could be performed within 7 min. The linear ranges were 10–35 μM, 35–150 μM for Lys; and 5–35 μM, 35–150 μM for His. The detection limits ( S/N = 3) were 0.3 μM for Lys and 1.0 μM for His, respectively. The proposed method was also successfully used for the determination of Lys in the oral pharmaceutical formulations. Copyright © 2012 John Wiley & Sons, Ltd.