Premium
CdS nanoparticles‐ enhanced chemiluminescence and determination of baicalin in pharmaceutical preparations
Author(s) -
Chen Xiaolan,
Tan Xinmei,
Wang Jianxiu
Publication year - 2012
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.2359
Subject(s) - baicalin , potassium ferricyanide , chemiluminescence , detection limit , chemistry , luminol , ferricyanide , nanoparticle , nuclear chemistry , absorbance , linear range , chromatography , inorganic chemistry , nanotechnology , materials science , high performance liquid chromatography
CdS nanoparticles (CdS NPs) of different sizes were synthesized by the citrate reduction method. It was found that CdS NPs could enhance the chemiluminescence (CL) of the luminol‐potassium ferricyanide system and baicalin could inhibit CdS NPs‐enhanced luminol‐potassium ferricyanide CL signals in alkaline solution. Based on this inhibition, a flow‐injection CL method was established for determination of baicalin in pharmaceutical preparations and human urine samples. Under optimized conditions, the linear range for determination of baicalin was 5.0 x 10 −6 to 1.0 x 10 −3 g/L. The detection limit at a signal‐to‐noise ratio of 3 was 1.7 x 10 −6 g/L. CL spectra, UV‐visible spectra and transmission electron microscopy (TEM) were used to investigate the CL mechanism. The method described is simple, selective and obviates the need of extensive sample pretreatment. Copyright © 2012 John Wiley & Sons, Ltd.