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Chemiluminescence determination of gemifloxacin based on diperiodatoargentate (III)‐sulphuric acid reaction in a micellar medium
Author(s) -
Zhao Fang,
Zhao Wenhui,
Xiong Wei
Publication year - 2012
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.2347
Subject(s) - gemifloxacin , chemiluminescence , chemistry , detection limit , relative standard deviation , bromide , sulfuric acid , pulmonary surfactant , micelle , chromatography , nuclear chemistry , inorganic chemistry , organic chemistry , aqueous solution , antibacterial agent , biochemistry , antibiotics
A novel flow‐injection chemiluminescence (FI‐CL) analysis method for the determination of gemifloxacin in the presence of cetyltrimethylammonium bromide (CTAB) surfactant micelles is described. Strong CL signal was generated during the reaction of gemifloxacin with diperiodatoargentate (III) in a sulfuric acid medium sensitized by CTAB. Under optimum experimental conditions, the CL intensity was linearly related to the concentration of gemifloxacin from 1.0 × 10 ‐9 to 3.0 × 10 ‐7 g/mL and the detection limit was 7.3 × 10 ‐10 g/mL (3σ). The relative standard deviation (RSD) was 1.7 % for a 3.0 × 10 ‐8 g/mL gemifloxacin solution (11 repeated measurements). The proposed method was successfully applied to the determination of gemifloxacin in pharmaceutical preparations and biological fluids. The possible mechanism of the CL reaction is also discussed briefly. Copyright © 2012 John Wiley & Sons, Ltd.