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Rapid methods of detecting the target molecule in immunohistology using a bioluminescence probe
Author(s) -
Wu Chun,
Wang KeYong,
Guo Xin,
Sato Masanori,
Ozaki Michitaka,
Shimajiri Shyohei,
Ohmiya Yoshihiro,
Sasaguri Yasuyuki
Publication year - 2012
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.2333
Subject(s) - bioluminescence , in vivo , labelling , molecular probe , immunohistochemistry , ex vivo , peroxidase , chemistry , antigen , antibody , microbiology and biotechnology , fluorescence , biology , biophysics , biochemistry , in vitro , enzyme , immunology , physics , dna , quantum mechanics
We demonstrate a novel rapid direct detection method for immunohistochemistry, using a bioluminescent probe. An anti‐CEA antibody‐fused far‐red bioluminescent protein can monitor the accumulation of this type of probe in tumour tissues. The bimodal spectrum ( λ max = 460 and 675 nm) of this bioluminescent probe is extremely stable under different conditions of pH and ion concentration. The sensitivity of our bioluminescent labelling was at the same level of enzymatic labelling, e.g. peroxidase, as an indirect system. Our novel technique is simple and can shorten the pretreatment time of paraffin sections to around 30 min. The utility of our bioluminescent labelling covers all imaging in vitro , in vivo and ex vivo , suggesting that our antibody‐fused bioluminescent probe has the potential to detect tumour antigens with a high sensitivity in routine immune histological examinations. Copyright © 2012 John Wiley & Sons, Ltd.