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Chemiluminescence of a cyclometallated iridium(III) complex and its application in the detection of cysteine
Author(s) -
Dong Yong Ping,
Shi Ming Juan,
Tong Bi Hai,
Zhang Qian Feng
Publication year - 2011
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.1365
Subject(s) - chemiluminescence , iridium , cysteine , luminescent measurements , chemistry , combinatorial chemistry , chromatography , luminescence , biochemistry , materials science , optoelectronics , catalysis , enzyme
Chemiluminescence (CL) of a cyclometallated iridium (III) complex {tris[1‐(2,6‐dimethylphenoxy)‐4‐(4‐chlorophenyl)phthalazine]iridium(III)} in the presence of potassium permanganate and oxalic acid is reported for the first time. Cysteine exhibits sufficient enhancing effect on the CL generated from the cyclometallated iridium(III) complex, which make it possible for the sensitive detection of cysteine using a flow‐injection–chemiluminescence (FI–CL) method. The optimum conditions for the chemiluminescence emission were investigated. Under the optimal condition, the linear range for the determination of cysteine was 1.0 × 10 –9 –5.0 × 10 –6 mol/L with a detection limit of 6.9 × 10 –10 mol/L. A relative standard deviation of 1.6% was obtained for eight replicate determinations. The mechanisms of CL are proposed and the emitting species was identified as the metal‐to‐ligand charge‐transfer (MLCT) excited states of the iridium complex. Copyright © 2011 John Wiley & Sons, Ltd.