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A highly sensitive and temporal visualization system for gaseous ethanol with chemiluminescence enhancer
Author(s) -
Arakawa Takahiro,
Ando Eri,
Wang Xin,
Kumiko Miyajima,
Kudo Hiroyuki,
Saito Hirokazu,
Mitani Tomoyo,
Takahashi Mitsuo,
Mitsubayashi Kohji
Publication year - 2011
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.1352
Subject(s) - luminol , chemiluminescence , chemistry , detection limit , eosin y , ethanol , hydrogen peroxide , chromatography , analytical chemistry (journal) , biochemistry , photocatalysis , catalysis
A two‐dimensional gaseous ethanol visualization system has been developed and demonstrated using a horseradish peroxidase–luminol–hydrogen peroxide system with high‐purity luminol solution and a chemiluminescence (CL) enhancer. This system measures ethanol concentrations as intensities of CL via the luminol reaction. CL was emitted when the gaseous ethanol was injected onto an enzyme‐immobilized membrane, which was employed as a screen for two‐dimensional gas visualization. The average intensity of CL on the substrate was linearly related to the concentration of standard ethanol gas. These results were compared with the CL intensity of the CCD camera recording image in the visualization system. This system is available for gas components not only for spatial but also for temporal analysis in real time. A high‐purity sodium salt HG solution (L‐HG) instead of standard luminol solution and an enhancer, eosin Y (EY) solution, were adapted for improvement of CL intensity of the system. The visualization of gaseous ethanol was achieved at a detection limit of 3 ppm at optimized concentrations of L‐HG solution and EY. Copyright © 2011 John Wiley & Sons, Ltd.