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Highly sensitive chemiluminescence determination of tenoxicam using a cerium(IV)–sodium hyposulphite system in micellar medium
Author(s) -
Chen Siyu,
Zhao Fang
Publication year - 2011
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.1346
Subject(s) - tenoxicam , chemiluminescence , chemistry , detection limit , chromatography , cerium , piroxicam , inorganic chemistry , medicine , alternative medicine , pathology
A simple, rapid and precise flow‐injection–chemiluminescence (FI–CL) method is presented for the determination of tenoxicam in pharmaceutical preparations and biological samples. The method is based on the weak chemiluminescence signal arising from the reaction of cerium(IV) in a nitric acid medium with sodium hyposulphite being significantly increased by tenoxicam in the presence of sodium dodecyl benzene sulphonate. Several experimental parameters affecting the CL reaction were examined and optimized systematically. Under the optimum conditions, the CL intensity was proportional to the concentration of tenoxicam in the range 7.0 × 10 –11 –5.0 × 10 –8 g/mL. The detection limit was 2.3 × 10 –11 g/mL tenoxicam and the relative standard deviation (RSD) was 2.1% for 1.0 × 10 –9 g/mL tenoxicam solution ( n = 11). The proposed method was applied to the determination of tenoxicam in pharmaceutical preparations, serum and human urine, with satisfactory results. The possible mechanism of the chemiluminescence reaction is also briefly discussed. Copyright © 2011 John Wiley & Sons, Ltd.