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Flow‐injection chemiluminescence determination of melamine in urine and plasma
Author(s) -
Tang Xiaoshuang,
Shi Xiyan,
Tang Yuhai,
Yue Zhongjin,
He Qiqi
Publication year - 2011
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.1341
Subject(s) - melamine , chemiluminescence , detection limit , luminol , chromatography , chemistry , urine , extraction (chemistry) , flow injection analysis , analytical chemistry (journal) , organic chemistry , biochemistry
A novel flow‐injection chemiluminescence method for the determination of melamine in urine and plasma was developed. It was found that melamine can remarkably enhance chemiluminescence emission from the luminol–K 3 Fe(CN) 6 system in an alkaline medium. Under the optimum conditions, chemiluminescence intensity had a good linear relationship with the concentration of melamine in the range 9.0 × 10 –9 –7.0 × 10 –6 g/mL, with a correlation coefficient of 0.9992. The detection limit (3 σ ) was 3.5 ng/mL. The method has been applied to determine the concentration of melamine in samples using liquid–liquid extraction. Average recoveries of melamine were 102.6% in urine samples and 95.1% in plasma samples. The method provided a reproducible and stable approach for the sensitive detection of melamine in urine and plasma samples. Copyright © 2011 John Wiley & Sons, Ltd.