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Highly sensitive spectrofluorimetic determination of rutin based on its activated effect on a multi‐enzyme redox system
Author(s) -
Chen YaHong,
Tian FengShou
Publication year - 2011
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.1325
Subject(s) - rutin , detection limit , chemistry , relative standard deviation , redox , linear range , chromatography , calibration curve , linear relationship , fluorescence , analytical chemistry (journal) , inorganic chemistry , biochemistry , mathematics , antioxidant , statistics , physics , quantum mechanics
ABSTRACT A highly sensitive and simple spectrofluorimetric method for the determination of rutin, based on its activated effect on a haemoglobin‐catalysed reaction, was developed. Under optimum conditions, the concentration of rutin was linear, with decreased fluorescence ( ΔF ) of the system under optimal experimental conditions. The calibration graph was linear in the range 1.0 × 10 –7 –3.0 × 10 –5 mol/L, with a detection limit of 7.0 × 10 –8 mol/L. The relative standard deviation (RSD) was 3.26% for 11 determinations of 1.0 × 10 –5 mol/L. This method was used for the determination of rutin in pharmaceuticals with satisfactory results. Copyright © 2011 John Wiley & Sons, Ltd.