Highly sensitive spectrofluorimetic determination of rutin based on its activated effect on a multi‐enzyme redox system | Zendy

Chen YaHong | Zendy; Tian FengShou | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Premium

Highly sensitive spectrofluorimetic determination of rutin based on its activated effect on a multi‐enzyme redox system

Author(s) -

Chen YaHong,

Tian FengShou

Publication year - 2011

Publication title -

luminescence

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.428

H-Index - 45

eISSN - 1522-7243

pISSN - 1522-7235

DOI - 10.1002/bio.1325

Subject(s) - rutin , detection limit , chemistry , relative standard deviation , redox , linear range , chromatography , calibration curve , linear relationship , fluorescence , analytical chemistry (journal) , inorganic chemistry , biochemistry , mathematics , antioxidant , statistics , physics , quantum mechanics

A highly sensitive and simple spectrofluorimetric method for the determination of rutin, based on its activated effect on a haemoglobin‐catalysed reaction, was developed. Under optimum conditions, the concentration of rutin was linear, with decreased fluorescence ( ΔF ) of the system under optimal experimental conditions. The calibration graph was linear in the range 1.0 × 10 –7 –3.0 × 10 –5 mol/L, with a detection limit of 7.0 × 10 –8 mol/L. The relative standard deviation (RSD) was 3.26% for 11 determinations of 1.0 × 10 –5 mol/L. This method was used for the determination of rutin in pharmaceuticals with satisfactory results. Copyright © 2011 John Wiley & Sons, Ltd.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research