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Gold nanoparticles‐based fluorescence enhancement of the terbium–levofloxacin system and its application in pharmaceutical preparations
Author(s) -
Lee Sang Hak,
Wabaidur Saikh Mohammad,
Alothman Zeid Abdullah,
Alam Seikh Mafiz
Publication year - 2011
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.1311
Subject(s) - detection limit , terbium , levofloxacin , colloidal gold , fluorescence , chemistry , aqueous solution , analyte , nanoparticle , analytical chemistry (journal) , nuclear chemistry , materials science , chromatography , ion , nanotechnology , optics , organic chemistry , biochemistry , physics , antibiotics
A sensitive fluorescence (FL) technique is proposed for the determination of levofloxacin (LVX). The method is based on the fact that the weak FL signal of the Tb(III)–LVX system is strongly enhanced in the presence of gold nanoparticles. Gold nanoparticles were prepared by the citrate reduction of HAuCl 4 and characterized by transmission electron microscopy (TEM). Levofloxacin and Tb(III) ion form a fluorescence complex in aqueous solution, and its maximum emission wavelength was found at 545 nm. Optimal conditions for the formation of the levofloxacin–Tb(III) complexes were studied. Levofloxacin was detected by measuring the FL intensity, which increases linearly with the concentration of LVX in the range 6.2 × 10 −10 –2.6 × 10 −8 mol/L. Recovery of the target analytes was > 96% with good quality parameters: linearity ( r 2 > 0.996), limit of detection (LOD) and limit of quantification (LOQ) values 2.1 × 10 −10 mol/L and 7.2 × 10 −10 mol/L, and run‐to‐run and day‐to‐day precisions with relative standard deviations (RSDs) around 3%. Thus, the proposed method can be successfully applied to the routine determination of levofloxacin in pharmaceutical preparations. Copyright © 2011 John Wiley & Sons, Ltd.