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Determination of clomipramine by flow‐injection analysis with acidic potassium permanganate–formic acid chemiluminescence detection
Author(s) -
Ji Zhongling,
Yao Xun,
Li Jianguo
Publication year - 2011
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.1307
Subject(s) - potassium permanganate , chemiluminescence , chemistry , formic acid , detection limit , reagent , chromatography , relative standard deviation , clomipramine , linear range , potassium , flow injection analysis , permanganate , correlation coefficient , inorganic chemistry , organic chemistry , pharmacology , medicine , statistics , mathematics
A sensitive and simple chemiluminescent (CL) method for the determination of clomipramine has been developed by combining the flow‐injection analysis (FIA) technique, which is based on the CL intensity generated from the redox reaction of potassium permanganate (KMnO 4 )–formic acid in sulphuric acid (H 2 SO 4 ) medium. Under the optimum conditions, the linear range for the determination of clomipramine was 0.04–4 µg/mL, with a correlation coefficient of 0.9988 ( n = 10) and a detection limit of 0.008 µg/mL (3 σ ), and the relative standard deviation (RSD) for 2.0 µg/mL clomipramine ( n = 11) is 1.26%. The proposed method has been successfully applied to the determination of the studied clomipramine in pharmaceutical preparations. The possible reaction mechanism is discussed. Copyright © 2011 John Wiley & Sons, Ltd.