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Sodium azide as a specific quencher of singlet oxygen during chemiluminescent detection by luminol and Cypridina luciferin analogues
Author(s) -
Bancirova Martina
Publication year - 2011
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.1296
Subject(s) - chemistry , chemiluminescence , sodium azide , luminol , singlet oxygen , luciferin , hydrogen peroxide , reactive oxygen species , photochemistry , superoxide , oxygen , superoxide dismutase , azide , biochemistry , organic chemistry , antioxidant , enzyme , luciferase , transfection , gene
ABSTRACT Reactive oxygen species (ROS) are presently thought to play important role in an increasing number of the physiological and pathological processes in living organisms. Various chemiluminescent (CL) compounds have been studied in order to find suitable and specific probes for the detection of particular ROS species. The CL of luminol is known to be non‐specific and can be induced by various oxidants. Two Cypridina luciferin analogues, CLA and MCLA, have been used for the detection of ROS in vivo . CLAs are thought to emit light only when reacting with superoxide and singlet oxygen. It is possible to distinguish the particular ROS by using a specific quencher or scavenger, e.g. superoxide dismutase (SOD) or sodium azide (NaN 3 ). The CL reactions of luminol (3‐aminophthalhydrazide), CLA [2‐methyl‐6‐phenyl‐3,7‐dihydroimidazo(1,2 α ) pyrazin‐3‐one] and MCLA [2‐methyl‐6‐( p ‐methoxyphenyl)‐3,7‐dihydroimidazo(1,2 α ) pyrazin‐3‐one] were studied in three hydrogen peroxide decomposition systems (H 2 O 2 –HRP; H 2 O 2 –CuSO 4 ; and H 2 O 2 –NaOCl). The measurements were carried out in phosphate buffer, pH 7.4, at 25 °C, using a luminometer (Fluoroskan Ascent FL and Sirius C). NaN 3 was used as the specific quencher of singlet oxygen. The results demonstrate that the proclaimed specifity of the CL of Cypridina luciferin analogues towards singlet oxygen has to be discussed. Copyright © 2011 John Wiley & Sons, Ltd.