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A label‐free immunoassay using eggshell membrane as matrix and poly(diallyldimethylammonium chloride) as light‐scattering enhancer
Author(s) -
Tang Jieli,
Han Lu,
Yu Yanhui,
Kang Jing,
Zhang Yihua
Publication year - 2011
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.1287
Subject(s) - eggshell membrane , detection limit , glutaraldehyde , chemistry , membrane , chromatography , immunoassay , matrix (chemical analysis) , eggshell , scattering , analytical chemistry (journal) , biochemistry , biology , physics , optics , ecology , antibody , immunology
A label‐free immunoassay system using eggshell membrane as a matrix was developed. A common spectrofluorometer was used to collect light‐scattering signals. The rabbit anti‐human IgG (Ab) was first immobilized on the eggshell membrane with glutaraldehyde. Then, based on the immunoreactions and electrostatic interaction, the target human IgG antigen (Ag) and poly(diallyldimethylammonium chloride) (PDDA) were captured on the eggshell membrane. It was found that the light‐scattering signal resulting from the PDDA immunotargeted on modified eggshell membrane was related to the concentration of target antigen. Under the optimal conditions, the light scattering intensity is directly proportional to the concentration of Ag in the range of 5.00–500 ng/mL ( r = 0.995) with the limit of detection of 2.31 ng/mL [signal:noise ratio (S:N) = 3]. The proposed method was successfully applied to the determination of IgG in human serum, and the results were in agreement with those obtained by a general immunonephelometric method. Copyright © 2011 John Wiley & Sons, Ltd.