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A novel chemiluminescent flow‐injection analysis of transferrin by its reduction of the luminol—hydrogen peroxide reaction catalysed by meso‐tetra‐(3‐methoxyl‐4‐hydroxyl) phenyl manganese porphyrin
Author(s) -
Wu Dan,
Han Yanyan,
Wei Qin,
Zhao Yanfang,
Mao Kexia,
Cai Yanyan,
Li Ru,
Dai Yuxue
Publication year - 2011
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.1286
Subject(s) - chemiluminescence , luminol , chemistry , hydrogen peroxide , manganese , flow injection analysis , detection limit , porphyrin , transferrin , tetra , peroxide , catalysis , photochemistry , inorganic chemistry , nuclear chemistry , medicinal chemistry , chromatography , organic chemistry , biochemistry
Based on the inhibition effect of transferrin (Tf) on the reaction of the luminol–hydrogen peroxide (H 2 O 2 ) chemiluminescence (CL) system, catalysed by meso‐tetra‐(3‐methoxyl‐4‐hydroxyl) phenyl manganese porphyrin (MnP) as a mimetic enzyme of peroxides, a sensitive flow‐injection CL method has been developed for the determination of Tf in an alkaline medium. The CL reaction was carefully investigated by examining the variations of reaction conditions. Under optimum conditions, the linear range for the determination of transferrin was 0.04–20.0 μg/mL and the detection limit was 1.62 ng/mL. This proposed method was sensitive, convenient and simple, and has been successfully applied to the determination of transferrin in a serum sample. Copyright © 2011 John Wiley & Sons, Ltd.