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Direct chemiluminescence determination of penicillin G potassium and a chemometrical optimization approach
Author(s) -
Sorouraddin Mohammad H.,
Iranifam Mortaza,
Naseri Abdolhossein,
FadakarSardroud Mohammad,
GharariAlibabalou Hossein
Publication year - 2011
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.1285
Subject(s) - chemiluminescence , penicillin , chemistry , chromatography , potassium , biochemistry , antibiotics , organic chemistry
A highly selective and simple chemiluminescence (CL) method for determination of penicillin G potassium (PGK) was developed. In the proposed method, CL was elicited from PGK upon its oxidation with H 2 O 2 . The light emission was enhanced in the presence of N ‐cetyl‐ N , N , N ‐trimethylammonium bromide (CTMAB). An experimental design, central composite design (CCD), was used to realize the optimized variables, including pH, surfactant (CTMAB) and H 2 O 2 concentrations. Under optimum condition, the calibration graph was linear in the range 3.3 × 10 −3 –3.3 × 10 −1 mmol/L, with a detection limit of 8.8 × 10 −4 mmol/L for PGK. The precision was calculated by analysing samples containing 1.6 × 10 −1 mmol/L PGK ( n = 5) and the relative standard deviation (RSD) was 1.40%. The utility of this method was demonstrated by determining PGK in pharmaceutical formulations for injection. The proposed method was validated by a reference method. Copyright © 2011 John Wiley & Sons, Ltd.