Premium
Characterization of the superoxide anion radical scavenging activity by tetracycline antibiotics in aprotic media
Author(s) -
Kładna Aleksandra,
Kruk Irena,
Michalska Teresa,
Berczyński Paweł,
AboulEnein Hassan Y.
Publication year - 2011
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.1283
Subject(s) - oxytetracycline , chlortetracycline , chemistry , tetracycline , tiron , doxycycline , superoxide , antibiotics , tetracycline hydrochloride , antioxidant , tetracycline antibiotics , nuclear chemistry , biochemistry , enzyme
The tetracycline family antibiotics are widely used as human and veterinary treatments. The drugs are effective as antibiotics and also show antimicrobial and non‐microbial action. However, the antioxidant properties of tetracyclines have not been characterized in aprotic media. To better understand their biological functions, the in vitro superoxide anion radical ( O 2 • ¯ ) scavenging activities of tetracycline, chlortetracycline, oxytetracycline, doxycycline and methacycline were characterized, along with a very efficient O 2 • ¯scavenger, tiron, in dimethyl sulphoxide (DMSO), using ultra‐weak chemiluminescence (CL). We found that tetracycline, chlortetracycline and doxycycline efficiently inhibited CL from the O 2 • ¯ ‐generating system at concentration levels of 0.02–1.0 mmol/L. Methacycline and oxytetracycline were the O 2 • ¯ scavengers at concentration levels of 0.01–0.1 mmol/L, whereas when their concentration was lowered the drugs were capable of generating O 2 • ¯ , leading to CL enhancement. For all the data obtained in this study, the scavenging activity for the compounds tested decreased in the following order: tetracycline > doxycycline > chlortetracycline > tiron methacycline > oxytetracycline. These results indicate that the tetracycline drugs directly alter O 2 • ¯redox chemistry in aprotic media. Copyright © 2011 John Wiley & Sons, Ltd.