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High‐sensitivity spectrofluorimetric determination of tiopronin based on inhibition of hemoglobin
Author(s) -
Chen YaHong,
Tian FengShou,
Zhang GaoFeng
Publication year - 2010
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.1255
Subject(s) - tiopronin , detection limit , chemistry , chromatography , calibration curve , hemoglobin , linear range , relative standard deviation , biochemistry , pharmacology , biology
A highly sensitive and simple spectrofluorimetric method for the determination of tiopronin based on its inhibitory effect on the hemoglobin‐catalyzed reaction of H 2 O 2 and l ‐tyrosine was developed. The concentration of tiopronin is linear with decreased fluorescence (Δ F ) of the system under the optimal experimental conditions. The calibration graph is linear in the range 1.23 × 10 −8 to 3.06 × 10 −5 mol L −1 with a detection limit of 6.13 × 10 −9 mol L −1 . The relative standard deviation was 4.38% for 11 determinations of 6.13 × 10 −6 mol L −1 . This method can be used for the determination of tiopronin in pharmaceuticals with satisfactory results. Copyright © 2010 John Wiley & Sons, Ltd.