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Development of chemiluminescence method for determination of 10‐hydroxycamptothecin based on luminol–[Ag(HIO 6 ) 2 ] 5− reaction in alkaline solution
Author(s) -
Sun Hanwen,
Chen Peiyun,
Shi Shasha,
Li Liqing
Publication year - 2010
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.1238
Subject(s) - chemiluminescence , luminol , chemistry , detection limit , luminescence , analytical chemistry (journal) , chromatography , materials science , optoelectronics
A novel chemiluminescence (CL) method was developed for the determination of 10‐hydroxycamptothecin(HCPT) based on the CL reaction between [Ag(HIO 6 ) 2 ] 5− and luminol in alkaline solution. CL emission of Ag(III) complex–luminol in alkaline medium was very different from that in acidic medium. A possible mechanism of enhanced CL emission was suggested. The enhanced effect of HCPT on CL emission of the [Ag(HIO 6 ) 2 ] 5− –luminol system was found. The enhanced degree of CL emission was proportional to HCPT concentration. The effect of the reaction conditions on CL emission was examined. Under optimal conditions, the limit of detection was 6.5 × 10 −9 g mL −1 . The proposed method was applied for the determination of HCPT in real samples with the recoveries of 93.2–109% with the RSD of 1.7–3.3%. Copyright © 2010 John Wiley & Sons, Ltd.