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Simple and sensitive spectrofluorimetric method for the determination of pregabalin in capsules through derivatization with fluorescamine
Author(s) -
Walash M. I.,
Belal F.,
ElEnany N.,
ElMaghrabey M. H.
Publication year - 2010
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.1235
Subject(s) - fluorescamine , chromatography , derivatization , pregabalin , detection limit , chemistry , fluorescence , derivative (finance) , analytical chemistry (journal) , high performance liquid chromatography , psychology , physics , financial economics , psychotherapist , economics , quantum mechanics
A new, simple and sensitive spectrofluorimetric method has been developed for the determination of pregabalin (PG) in capsules. The method is based on the reaction between pregabalin and fluorescamine in borate buffer solution of pH 10 to give a highly fluorescent derivative that is measured at 487 nm after excitation at 390 nm. The different experimental parameters affecting the development and stability of the reaction product were carefully studied and optimized. The fluorescence intensity concentration plot was rectilinear over the range of 0.01–0.3 µg mL −1 with a lower detection limit of 0.0017 µg mL −1 and limit of quantitation of 0.005 µg mL −1 . The developed method was successfully applied to the analysis of the drug in its commercial capsules. The mean percentage recovery of PG in its capsule was 99.93±1.24 ( n = 3). Statistical comparison of the results with those of the comparison method revealed good agreement and proved that there was no significant difference in the accuracy and precision of the two methods. A proposed reaction pathway was postulated. Copyright © 2010 John Wiley & Sons, Ltd.