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Terbium‐sensitized fluorescence method for the determination of deferasirox in biological fluids and tablet formulation
Author(s) -
Manzoori Jamshid L.,
Jouyban Abolghasem,
Amjadi Mohammad,
PanahiAzar Vahid,
Tamizi Elnaz,
VaezGharamaleki Jalil
Publication year - 2011
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.1218
Subject(s) - deferasirox , detection limit , reproducibility , terbium , chemistry , linear range , urine , excitation wavelength , chromatography , analytical chemistry (journal) , fluorescence , medicine , optics , physics , ion , biochemistry , thalassemia , organic chemistry
Abstract A novel, rapid and sensitive spectroflurimetric method was developed and validated for the determination of deferasirox in urine, serum and tablet samples based on sensitization of terbium fluorescence. The excitation and emission wavelengths were 328 and 545 nm, respectively. The optimum conditions for the determination of deferasirox were investigated considering the effects of various parameters. The method was quantitatively evaluated in terms of linearity, recovery, reproducibility and limit of detection. Under the optimal conditions, the fluorescence intensities were linear with the concentration of deferasirox in the range of 5 × 10 −9 to 5×10 −6 mol L −1 , with a detection limit of 1.5 × 10 −9 mol L −1 and a relative standard deviation of 1.1–2.3%. Linearity, reproducibility, recovery and limit of detection made the method suitable for determination of deferasirox in urine, serum and tablets samples. Copyright © 2010 John Wiley & Sons, Ltd.