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Radical scavenging ability of some compounds isolated from Piper cubeba towards free radicals
Author(s) -
AboulEnein Hassan Y.,
Kładna Aleksandra,
Kruk Irena
Publication year - 2011
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.1209
Subject(s) - chemistry , radical , dpph , superoxide , electron paramagnetic resonance , antioxidant , hydroxyl radical , superoxide dismutase , nuclear chemistry , chemiluminescence , scavenging , superoxide radical , photochemistry , organic chemistry , medicinal chemistry , enzyme , nuclear magnetic resonance , physics
The purpose of this study was to identify the antioxidant activity of 16 compounds isolated from Piper cubeba (CNCs) through the extent of their capacities to scavenge free radicals, hydroxyl radical (HO • ), superoxide anion radical () and 2,2‐diphenyl‐1‐picrylhydrazyl radical (DPPH • ), in different systems. Electron paramagnetic resonance (EPR) and 5,5‐dimethyl‐1‐pyrroline‐ N ‐oxide, DMPO, as the spin trap, and chemiluminescence techniques were applied. Using the Fenton‐like reaction [Fe(II) + H 2 O 2 ], CNCs were found to inhibit DMPOOH radical formation ranging from 5 to 57% at 1.25 mmol L −1 concentration. The examined CNCs also showed a high DPPH antiradical activity (ranging from 15 to 99% at 5 mmol L −1 concentration). Furthermore, the results indicated that seven of the 16 tested compounds may catalyse the conversion of superoxide radicals generated in the potassium superoxide/18‐crown‐6 ether system, thus showing superoxide dismutase‐like activity. The data obtained suggest that radical scavenging properties of CNCs might have potential application in many plant medicines. Copyright © 2010 John Wiley & Sons, Ltd.