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The relationship between exhaled carbon monoxide and human neutrophil function in the Japanese general population
Author(s) -
Mikami Makoto,
Takahashi Ippei,
Matsuzaka Masashi,
Danjo Kazuma,
Yamai Kiyonori,
Inoue Ryo,
Iwane Kaori,
Umeda Takashi,
Nakaji Shigeyuki
Publication year - 2011
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.1199
Subject(s) - carbon monoxide , population , function (biology) , chemistry , medicine , biology , biochemistry , environmental health , catalysis , evolutionary biology
We have evaluated the relationship between exhaled carbon monoxide (CO) level and neutrophil‐related functions such as reactive oxygen species (ROS) production capability, phagocytic activity and serum opsonic activity in the general population. Serum opsonic activity was determined by measuring the effects of serum on neutrophil ROS production capability using lucigenin‐ and luminol‐dependent chemiluminescence (LgCL, LmCL). LgCL is associated with the detection of O 2 − , whereas LmCL mainly detects H 2 O 2 and HOCl, which are higher reactive oxygen radicals. In females, exhaled CO level was found to have positive associations with ROS production capability and LgCL. However, the opposite tendency was seen between exhaled CO level and LmCL in both genders. This result suggests that neutrophil ROS production in females may have contributed to oxidative stress, which led to the increases in intrinsic CO and exhaled CO consequently. Such changes then may have inhibited the process of changing reactive oxygen radicals into higher oxidizing potential levels. Copyright © 2010 John Wiley & Sons, Ltd.