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Spectrofluorimetric determination of folic acid in tablets and urine samples using 1,10‐phenanthroline‐terbium probe
Author(s) -
Manzoori Jamshid L.,
Jouyban Abolghasem,
Amjadi Mohammad,
Soleymani Jafar
Publication year - 2011
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.1191
Subject(s) - terbium , chemistry , phenanthroline , detection limit , buffer solution , fluorescence , reagent , chromatography , folic acid , analytical chemistry (journal) , urine , dosage form , quenching (fluorescence) , fluorescence spectrometry , standard addition , inorganic chemistry , ion , organic chemistry , biochemistry , medicine , physics , quantum mechanics
A new spectrofluorimetric method was reported for the determination of folic acid (FA), based on its quenching effect on the fluorescence intensity of Tb 3+ –1,10‐phenanthroline complex as a fluorescent probe. The quenched fluorescence intensity at an emission wavelength of 545 nm was proportional to the concentration of FA in Tris–HCl buffer solution of pH 6.2. The effects of pH, time, order of addition of reagents, temperature and concentrations of Tb 3+ , buffer and 1,10‐phenanthroline were investigated and optimized. The linear range for the determination of FA was 0.01–1.1 mg/L. The detection limit was 0.003 mg/L and the relative standard deviation for replicated determination of 1 mg/L of folic acid was 1.2%. This method was simple, practical and relatively free from interference effects. It was successfully applied to assess FA in pharmaceutical tablets and urine samples. Copyright © 2010 John Wiley & Sons, Ltd.