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Development of a novel fluorometric assay for nitric oxide utilizing sesamol and its application to analysis of nitric oxide‐releasing drugs
Author(s) -
Abe Seiji,
Nakabayashi Shigeo,
Murayama JunIchiro,
Sano Yoshihiro,
Ohno KenIchi,
Maeda Masako,
Arakawa Hidetoshi
Publication year - 2010
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.1180
Subject(s) - sesamol , nitric oxide , chemistry , detection limit , substrate (aquarium) , combinatorial chemistry , radical , pharmacology , fluorescence , chromatography , antioxidant , biochemistry , organic chemistry , medicine , biology , ecology , physics , quantum mechanics
Nitric oxide (NO) is related to various physiological effects as well as to numerous diseases caused by accentuation of NO production. Measurement of NO in cells and tissues is difficult as NO readily reacts with other molecules; furthermore, its half‐life as a radical is fleeting. Currently, many NO pharmaceuticals are marketed as therapeutic agents for ischemic disease. Consequently, the identification of NO radicals and determination of generation rate from pharmaceuticals is very important when the effect of the medicinal supply is estimated. In this study, we developed a fluorometric assay for NO employing sesamol (3,4‐methylenedioxyphenol) as a fluorometric substrate. Sesamol is converted to a fluorescent derivative (ex. 365 nm, em. 447 nm), which is dimmer in the presence of NO. The detection limit of NO with this method is 400 fmol; moreover, NO generated from drugs can be measured. Copyright © 2009 John Wiley & Sons, Ltd.