Open AccessFrom Luc and Phot genes to the hospital bed
Author(s) -
Campbell Anthony K.,
SalaNewby Graciela,
Aston Paul J.,
Kalsheker Noor,
Kishi Yoshito,
Shimomura Osamu
Publication year - 1990
Publication title -
journal of bioluminescence and chemiluminescence
Language(s) - English
Resource type - Journals
eISSN - 1099-1271
pISSN - 0884-3996
DOI - 10.1002/bio.1170050209
Subject(s) - primer (cosmetics) , gene , cloning (programming) , polymerase , plasmid , luciferase , aequorea victoria , biology , polymerase chain reaction , microbiology and biotechnology , bioluminescence , computational biology , chemistry , biochemistry , genetics , gene expression , transfection , organic chemistry , computer science , programming language
The Luc gene from the firefly Photinus pyralis has been isolated by cloning it in pcDV1 PL plasmid primer and Honjo linker and the Phot gene isolated from Aequorea victoria using the polymerase chain reaction. A method has been established using SP6 RNA polymerase for transcribing and translating bioluminescent genes in vitro. It should now be possible to engineer these genes to measure intracellular ATP and the covalent modification of proteins in single, live cells, providing unique insights into the molecular basis of disease.