Premium
Resonance Rayleigh‐scattering spectral method for the determination of some aminoglycoside antibiotics using CdTe quantum dots as a probe
Author(s) -
Wang Lei,
Peng Juanjuan,
Liu Zhengwen,
He Youqiu
Publication year - 2010
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.1170
Subject(s) - rayleigh scattering , aminoglycoside , quantum dot , scattering , amikacin , chemistry , resonance (particle physics) , cadmium telluride photovoltaics , analytical chemistry (journal) , molecular physics , atomic physics , physics , optics , antibiotics , chromatography , optoelectronics , biochemistry
Abstract A novel method is used for the determination of some aminoglycoside antibiotics (AGs) such as etimicin (ETM), isepamicin (ISP) and amikacin (AMK). It is based on the resonance Rayleigh scattering (RRS) intensities enhanced by AGs‐induced CdTe quantum dots aggregation. Under the optimum conditions, the increments in RRS intensity were directly proportional to the concentration of AGs in certain ranges. At the same time, the second‐order scattering, the frequency‐doubling scattering and the frequency‐trebling scattering intensities were also enhanced and their increments were proportional to the concentration of AGs. Among them, the RRS method had the highest sensitivity; the linear ranges and detection limits for ETM, ISP and AMK were 0.085–7.2, 0.0067–1.2, 0.017–6.0 and 0.025, 0.0051, 0.0020 μg mL −1 . This method was applied to the measurement of AGs in human serum and urine with satisfactory results. In addition, the reaction mechanism and the reasons for the enhancement of RRS are discussed using fluorescence, RRS, transmission electron microscope technology and quantum chemistry method. Copyright © 2009 John Wiley & Sons, Ltd.