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A flow‐injection chemiluminescent method for the evaluation of the antioxidant activity of 5′‐nucleotides
Author(s) -
Wang Zhouping,
Xu Huan,
Qian Jia,
Ye Jing,
Yang Zhen,
Sun Huachao,
Shi Yonghui
Publication year - 2009
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.1149
Subject(s) - chemiluminescence , chemistry , nucleotide , antioxidant , flow injection analysis , flow (mathematics) , chromatography , biochemistry , detection limit , mechanics , physics , gene
In the present work, a simple chemiluminescence (CL) method coupled with flow‐injection analysis for the evaluation of antioxidant activity of 5′‐nucleotides (5′‐AMP, 5′‐CMP, 5′‐GMP, 5′‐UMP) was proposed. It is based on inhibition effect of the studied substances on CL emission of luminol–potassium ferricyanide–pyrogallol. Experiments were performed to evaluate the nature of the inhibition by 5′‐nucleotides of the CL reaction and their antioxidant activities. Based on the experimental results, it was observed that 5′‐nucleotides are available antioxidants that could effectively scavenge superoxide anion free radicals in a concentration‐dependent way. This will provide a basis for further development of the use of nucleotides. Copyright © 2009 John Wiley & Sons, Ltd.