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The fluorescence enhancement of quercetin–nucleic acid system and the analytical application
Author(s) -
Liu Yuan,
Wu Xia,
Zhou Haiping,
Liu Xiaoyu,
Zhang Fengju,
Yang Jinghe
Publication year - 2009
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.1128
Subject(s) - nucleic acid , quercetin , chemistry , fluorescence , dna , detection limit , nucleic acid quantitation , chromatography , biochemistry , physics , quantum mechanics , antioxidant
Nucleic acid can greatly enhance the fluorescence intensity of quercetin in HMTA‐HCl (pH 5.5) buffer. The enhanced intensity is in proportion to the concentration of nucleic acids in the range 5.0 × 10 −9 to 1.0 × 10 −6 g/mL for fsDNA, 5.0 × 10 −9 to 7.0 × 10 −7 g/mL for ctDNA and 5.0 × 10 −9 to 1.0 × 10 −6 g/mL for yRNA, and their detection limits (S/N = 3) are 3.5 × 10 −9 , 7.8 × 10 −10 and 2.6 × 10 −9 g/mL, respectively. In comparison with most reported fluorescent probes for the determination of nucleic acids, the proposed probe has higher sensitivity and lower toxicity. The interaction investigation indicates that quercetin binds with double‐strand DNA in groove binding mode, resulting in fluorescence enhancement of this system. Copyright © 2009 John Wiley & Sons, Ltd.