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Determination of nucleic acid by [tetra‐(3‐methoxy‐4‐hydroxyphenyl)]–Tb 3+ porphyrin as the fluorescence spectral probe in bis(2‐ethylhexyl)sulfosuccinate sodium salt micelle system
Author(s) -
Chen Xin,
Wei Qin,
Han Yanyan,
Sun Shuting,
Wu Dan,
Du Bin
Publication year - 2009
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.1102
Subject(s) - porphyrin , nucleic acid , chemistry , fluorescence , buffer solution , micelle , dna , sodium , nuclear chemistry , inorganic chemistry , aqueous solution , photochemistry , chromatography , organic chemistry , biochemistry , physics , quantum mechanics
A new system for the determination of nucleic acid by rare earth metallic porphyrin of [tetra‐(3‐methoxy‐4‐hydroxyphenyl)]–Tb 3+ [T(3‐MO‐4HP)–Tb 3+ ] porphyrin as fluorescence spectral probe has been developed in this paper. Nucleic acid can enhance the fluorescence intensity of the T(3‐MO‐4HP)–Tb 3+ porphyrin in the presence of bis(2‐ethylhexyl)sulfosuccinate sodium salt(AOT) micelle. In pH 8.00 Tris–HCl buffer solution, under optimum conditions, the enhanced fluorescence intensity is in proportion to the concentration of nucleic acids in the range of 0.05–3.00 µg mL −1 for calf thymus DNA (ct DNA) and 0.03–4.80 µg mL −1 for fish sperm DNA(fs DNA). Their detection limits are 0.03 and 0.01 µg mL −1 , respectively. In addition, the binding interaction mechanism between T(3‐MO‐4HP)–Tb 3+ porphyrin and ct DNA is also investigated by resonance scattering and fluorescence spectra. The maximum binding number is calculated by molar ratio method. The new system can be used for the determination of nucleic acid in pig liver, yielding satisfactory results. Copyright © 2009 John Wiley & Sons, Ltd.