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A highly sensitive fluorescent probe for tiopronin based on the cleavage of 2, 4‐dinitrobenzenesulfonate
Author(s) -
Wang Liping,
Yang XiaoFeng,
Xu Hongmei,
Zhao Minglei
Publication year - 2009
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.1100
Subject(s) - tiopronin , fluorescence , detection limit , absorbance , chemistry , chromatography , fluorescein , aqueous solution , cleavage (geology) , materials science , organic chemistry , optics , medicine , physics , composite material , fracture (geology) , pharmacology
A highly sensitive fluorogenic probe for tiopronin was proposed. 2,4‐Dinitrobenzenesulfonyl‐fluorescein (I) is an almost nonfluorescent compound. Upon mixing with tiopronin in aqueous solution, the 2,4‐dinitrobenzenesulfonyl group of I was efficiently removed and its parent dye fluorescein was released, hence leading to dramatic increases in both fluorescence and absorbance of the reaction mixture. Under optimal conditions, the fluorescence increase is linear with tiopronin concentration in the range 5.0–600 ng mL −1 , with a detection limit of 1.5 ng mL −1 (3 σ ). The proposed method has been successfully applied to tiopronin determination in pharmaceutical preparations and in spiked human urine samples. Copyright © 2009 John Wiley & Sons, Ltd.