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Analytical quantification of low‐density lipoprotein using europium tetracycline indicator
Author(s) -
De Oliveira Silva Flávia Rodrigues,
Monteiro Andrea Moreira,
Neto Antônio M. Figueiredo,
Gidlund Magnus,
Junior Nilson Dias Vieira,
Courrol Lilia Coronato
Publication year - 2009
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.1094
Subject(s) - europium , chemistry , low density lipoprotein , tetracycline , luminescence , aqueous solution , lipoprotein , cholesterol , biochemistry , materials science , optoelectronics , organic chemistry , ion , antibiotics
Low‐density lipoprotein (LDL) is known as ‘bad’ cholesterol. If too much LDL circulates in the blood it can be retained in the walls of the arteries, causing atherosclerosis. In this paper we showed an alternative method to quantify LDL using the europium tetracycline (EuTc) indicator. The optical properties of the EuTc complex were investigated in aqueous solutions containing LDL. An enhancement was observed of the europium luminescence in the solutions with LDL compared those without the lipoprotein. A method to quantify the amount of LDL in a sample, based on EuTc enhanced luminescence, is proposed. The enhancement mechanism is also discussed. Copyright © 2009 John Wiley & Sons, Ltd.