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Flow‐injection determination of vitamin A in pharmaceutical formulations using Tris(2,2′‐bipyridyl)Ru(II)–Ce(IV) chemiluminescence detection
Author(s) -
Yaqoob Mohammad,
Waseem Amir,
Rishi Lubna,
Nabi Abdul
Publication year - 2009
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.1089
Subject(s) - chemiluminescence , detection limit , relative standard deviation , chemistry , flow injection analysis , chromatography , tris , dosage form , vitamin , correlation coefficient , coefficient of variation , analytical chemistry (journal) , mathematics , biochemistry , statistics
A flow injection method with chemiluminescence detection is reported for the determination of vitamin A. The method is based on the enhancement effect of vitamin A on chemiluminescence of tris(2,2′‐bipyridyl)Ru(II)–Ce(IV) in acidic medium. The proposed procedure is used to quantitate vitamin A in the range 1.0–100 × 10 −6 mol/L with a correlation coefficient of 0.9991 ( n = 9) and relative standard deviation in the range 1.2–2.3% ( n = 4). The limit of detection (3 × blank) was 8.0 × 10 −8 mol/L with a sample throughput of 100/h. The effect of common excipients used in pharmaceutical formulations and some clinically important compounds was also studied. The method was applied to determine vitamin A in pharmaceutical formulations and the results obtained were in reasonable agreement with the amount quoted. The results were compared using spectrophotometric method and no significant difference was found between the results of the two methods at 95% confidence limit. Copyright © 2009 John Wiley & Sons, Ltd.