A bioluminescent enzyme immunoassay for prostaglandin E 2  using Cypridina luciferase | Zendy

Wu Chun | Zendy; Irie Saki | Zendy; Yamamoto Shozo | Zendy; Ohmiya Yoshihiro | Zendy

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A bioluminescent enzyme immunoassay for prostaglandin E 2 using Cypridina luciferase

Author(s) -

Wu Chun,

Irie Saki,

Yamamoto Shozo,

Ohmiya Yoshihiro

Publication year - 2009

Publication title -

luminescence

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.428

H-Index - 45

eISSN - 1522-7243

pISSN - 1522-7235

DOI - 10.1002/bio.1086

Subject(s) - luciferase , bioluminescence , immunoassay , chemistry , enzyme , prostaglandin , prostaglandin e2 , prostaglandin e , arachidonic acid , cyclooxygenase , biochemistry , biology , endocrinology , antibody , immunology , transfection , gene

Prostaglandin E 2 is one of the major cyclooxygenase metabolites of arachidonic acid. We developed a competitive immunosorbent assay for prostaglandin E 2 utilizing a bioluminescent enzyme Cypridina luciferase. The prostaglandin E 2 amount could be quantified over the concentration ranging from 7.8 to 500 pg/mL. The amount of unlabeled prostaglandin E 2 required to displace 50% of the maximal binding of Cypridina luciferase‐labeled prostaglandin E 2 ( B / B 0 ) was approximately 35 pg/mL. The results show a great potential of Cypridina luciferase as a new labeling enzyme for enzyme‐linked immunosorbent assay. Copyright © 2009 John Wiley & Sons, Ltd.

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