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A bioluminescent enzyme immunoassay for prostaglandin E 2 using Cypridina luciferase
Author(s) -
Wu Chun,
Irie Saki,
Yamamoto Shozo,
Ohmiya Yoshihiro
Publication year - 2009
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.1086
Subject(s) - luciferase , bioluminescence , immunoassay , chemistry , enzyme , prostaglandin , prostaglandin e2 , prostaglandin e , arachidonic acid , cyclooxygenase , biochemistry , biology , endocrinology , antibody , immunology , transfection , gene
Abstract Prostaglandin E 2 is one of the major cyclooxygenase metabolites of arachidonic acid. We developed a competitive immunosorbent assay for prostaglandin E 2 utilizing a bioluminescent enzyme Cypridina luciferase. The prostaglandin E 2 amount could be quantified over the concentration ranging from 7.8 to 500 pg/mL. The amount of unlabeled prostaglandin E 2 required to displace 50% of the maximal binding of Cypridina luciferase‐labeled prostaglandin E 2 ( B / B 0 ) was approximately 35 pg/mL. The results show a great potential of Cypridina luciferase as a new labeling enzyme for enzyme‐linked immunosorbent assay. Copyright © 2009 John Wiley & Sons, Ltd.