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Development of a novel capillary electrophoresis chemiluminescence system for amino acid analysis
Author(s) -
Yin Dongguang,
Xie Chunjuan,
Zhang Li,
Liu Binghu,
Zhou Xiaozhou,
Wang Peng,
Wu Minghong
Publication year - 2008
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.1076
Subject(s) - chemiluminescence , peroxyoxalate , capillary electrophoresis , dansyl chloride , chemistry , amine gas treating , chromatography , aspartic acid , relative standard deviation , amino acid , detection limit , analytical chemistry (journal) , derivatization , organic chemistry , mass spectrometry , biochemistry
In the present study, a novel peroxyoxalate CE–CL system was developed to achieve high signal stability and sensitivity based on a design of a new interface including a new mixing mode and a new grounding electrode mode. Amino acids fluorescently tagged with dansyl chloride and naphthalene‐2,3‐dicarboxaldehyde(NDA) were used for the study. Experiment results show this new system is quite effective to separate and detect amine acid with high stability and resolute. The detection limits were 1.1 nmol/L for dansyl‐leucine (Leu) and 2.0 nmol/L for dansyl‐aspartic acid (Asp). The relative standard deviations of peak height and migration time were in the ranges of 2.3–3.8% and 1.2–1.5%, respectively. Copyright © 2008 John Wiley & Sons, Ltd.