Highly sensitive spectrofluorimetric determination of trace amounts of prulifloxacin using the aluminium(III)–prulifloxacin system

The fluorescence of the prulifloxacin (PUFX)–Al(III) system was investigated . Experiments indicated that the fluorescence intensity of prulifloxacin could be greatly enhanced by Al(III) and sensitized by sodium dodecylbenzene sulphonate (SDBS). Accordingly, a sensitive spectrofluorimetric method for the determination of prulifloxacin was established. While excited at 275 nm, the enhanced fluorescence intensity at 412 nm of the system (Δ F ) showed a good linear relationship with the concentration of prulifloxacin within the range 4.0 × 10 –8 –3.0 × 10 –6  mol/L. The regression equation was Δ F  = 9.83 + 10.8 × 10 7 c (mol/L); the correlation coefficient and detection limit (3 σ /k) were 0.99901 and 2.0 × 10 –8 mol/L, respectively. The proposed method has been successfully applied to determine prulifloxacin in real pharmaceutical samples. The luminescence mechanism of the system is also discussed in detail. Copyright © 2008 John Wiley & Sons, Ltd.