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A critical evaluation of a flow‐cell based on a liquid core waveguide for chemiluminescence measurements
Author(s) -
Sanchez Mariana A.,
Rocha Fábio R. P.
Publication year - 2008
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.1071
Subject(s) - chemiluminescence , luminol , detection limit , iodometry , hydrogen peroxide , hypochlorite , chemistry , luminescence , analytical chemistry (journal) , flow injection analysis , linear range , titration , chromatography , confidence interval , materials science , inorganic chemistry , optoelectronics , mathematics , organic chemistry , statistics
Liquid‐core waveguides (LCWs), devices that constrain the emitted radiation minimizing losses during the transport, are an alternative to maximize the amount of detected radiation in luminescence. In this work, the performance of a LCW flow‐cell was critically evaluated for chemiluminescence measurements, by using as model the oxidation of luminol by hydrogen peroxide or hypochlorite. An analytical procedure for hypochlorite determination was also developed, with linear response in the range 0.2–3.8 mg/L (2.7–51 µmol/L), a detection limit estimated as 8 µg/L (0.64 µmol/L) at the 99.7% confidence level and luminol consumption of 50 µg/determination. The coefficients of variation were 3.3% and 1.6% for 0.4 and 1.9 mg/L ClO − , respectively, with a sampling rate of 164 determinations/h. The procedure was applied to the analysis of Dakin's solution samples, yielding results in agreement with those obtained by iodometric titration at the 95% confidence level. Copyright © 2008 John Wiley & Sons, Ltd.