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Luminescent and hydrophilic LaF 3 –polymer nanocomposite for DNA detection
Author(s) -
Wang Lun,
Li Peng,
Wang Leyu
Publication year - 2008
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.1061
Subject(s) - luminescence , fluorescence , polymer , microemulsion , nanocomposite , nanocrystal , quenching (fluorescence) , dna , quantum dot , materials science , nanoparticle , cyclohexane , chemistry , nuclear chemistry , analytical chemistry (journal) , nanotechnology , chromatography , organic chemistry , optics , optoelectronics , pulmonary surfactant , biochemistry , physics
Luminescent LaF 3 –Ce 3+ /Tb 3+ nanocrystals have been successfully prepared via a simple wet chemical technique. For the next bioapplication, these nanoparticles dispersed in cyclohexane have also been functionalized with poly(St‐co‐MAA), based on a designed oil‐in‐water microemulsion system. These polymer‐coated nanospheres are water‐soluble and bioconjugable. Unlike semiconductor quantum dots, the as‐prepared lanthanum fluoride nanocrystals possess non‐size‐dependent emissions and completely stable photocycles. With functionalized LaF 3 nanospheres as fluorescence probes, a fluorescence method was developed for the rapid quantitative analysis of DNA, due to the quenching effect of fluorescence by the DNA. Under optimum conditions, the fluorescence intensity was proportional to the concentration of the introduced DNA over the range 2.5–35 µg/mL for calf thymus DNA (ctDNA) and 2.5–30 µg/mL for fish sperm DNA (fsDNA), respectively. Copyright © 2008 John Wiley & Sons, Ltd.