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Resonance light‐scattering enhancement effect of the protein–Y 3+ –TTA–SLS system and its analytical application
Author(s) -
Guo Changying,
Wu Xia,
Xu Wei,
Yang Jinghe
Publication year - 2008
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.1053
Subject(s) - thenoyltrifluoroacetone , chemistry , analytical chemistry (journal) , resonance (particle physics) , detection limit , buffer solution , scattering , sodium , chromatography , nuclear chemistry , optics , extraction (chemistry) , physics , organic chemistry , particle physics , solvent extraction
In this paper, a sensitive resonance light scattering (RLS) method for the determination of protein is reported. In the Tris–HCl (pH 7.50) buffer, protein enhanced the RLS intensity of the Y 3+ –2‐thenoyltrifluoroacetone (TTA)–sodium dodecyl sulphate (SLS) system. The enhanced RLS intensities were in proportion to the concentrations of proteins in the range 8.0 × 10 −9 –1.0 × 10 −5 g/mL for BSA, 1.0 × 10 –8 –1.0 × 10 −5 g/mL for HSA and 1.0 × 10 –8 –1.0 × 10 −6 g/mL for EA, and their detection limits were 5.0, 5.4 and 6.7 ng/mL, respectively. Actual samples were satisfactorily determined. The interaction mechanism was also studied. Copyright © 2008 John Wiley & Sons, Ltd.
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