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Preparation of aminated core‐shell fluorescent nanoparticles and their application to the synchronous fluorescence determination of γ ‐globulin
Author(s) -
Gao Feng,
Luo Fabao,
Yin Jun,
Wang Lun
Publication year - 2008
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.1051
Subject(s) - fluorescence , detection limit , triethoxysilane , chemistry , aqueous solution , nanoparticle , linear range , chromatography , photobleaching , micelle , nuclear chemistry , analytical chemistry (journal) , materials science , nanotechnology , organic chemistry , physics , quantum mechanics
Amino‐modified silica nanoparticles (FSNPs) doped with fluorescein isothiocyanate (FITC) were synthesized by using an aqueous core of reverse‐micelle microemulsion as the nanoreactor in an easy one‐pot method. Due to the FITC conjugating with (3‐aminopropyl)triethoxysilane (APTS), the nanoparticles prevent the FITC from leaching from the silica matrix when immersed in aqueous solution. SEM, FTIR, fluorescence lifetime, a photobleaching experiment and synchronous fluorescence spectra were used to characterize the FSNPs. The synchronous fluorescence signal of FSNPs was enhanced when trace amounts of γ ‐globulin ( γ ‐G) were added. Under the optimal experimental conditions, the enhanced fluorescence intensity ( ΔF ) was linear with the concentration of γ ‐G ( c ) in the range 0.3–4.8 µg/mL, with a detection limit of 0.04 µg/mL. The proposed method is simple, sensitive for the determination of trace amounts of γ ‐G and used to determine the content of γ ‐G in synthetic samples with satisfactory results. Copyright © 2008 John Wiley & Sons, Ltd.