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Development of a sensitive flow‐injection–chemiluminescence detection method for the determination of laevodopa
Author(s) -
Alam Seikh Mafiz,
Karim Mohammad Mainul,
Lee Sang Hak,
Wabaidur Saikh Mohammad,
Chung Hye Young,
Choi Jong Ha,
Kang Meea
Publication year - 2008
Publication title -
luminescence
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.428
H-Index - 45
eISSN - 1522-7243
pISSN - 1522-7235
DOI - 10.1002/bio.1039
Subject(s) - chemiluminescence , detection limit , relative standard deviation , chemistry , chromatography , correlation coefficient , flow injection analysis , analytical chemistry (journal) , urine , coefficient of variation , mathematics , biochemistry , statistics
A simple chemiluminometric method using flow injection has been developed for the determination of laevodopa, based on its sensitizing effect on the weak chemiluminescence (CL) reaction between Na 2 SO 3 and acidic KMnO 4 . Under optimum experimental conditions, the CL intensity was linearly related to the concentration of laevodopa from 3.4 × 10 –8 to 2.4 × 10 –5 mol/L and the detection limit was 1.1 × 10 –8 mol/L (s:n = 3). The relative standard deviation (RSD) of the proposed method calculated from 20 replicate injection of 3 × 10 –7 mol/L laevodopa was 3.3%. The correlation coefficient was 0.997. The method was successfully applied to the determination of laevodopa in commercial pharmaceutical formulations and spiked urine samples. Copyright © 2008 John Wiley & Sons, Ltd.